Black Band Disease Molecular Analyses
Bruce W. Fouke
fouke at uiuc.edu
Mon Nov 26 10:25:24 EST 2001
The article you referenced was a news brief written by EarthVision
summarizing a presentation that Professor Abigail Salyers (Illinois
Microbiology) and I made on our newly initiated coral black band
disease (BBD) research at the November 2001 Geological Society of
America meeting in Boston.
We have just submitted a manuscript summarizing this work to Applied
and Environmental Microbiology. In this manuscript, all of the
previous work on BBD and some other relevant diseases has been
thoroughly referenced. The manuscript includes full references to the
excellent series of previous studies by workers such as Richardson,
Rutzler, Santavy, Antonius, Carlton, Kuta, Schnell, Peters, Edmunds,
Goreau, Williams, Rohwer, and several others (a total of 77
In our pilot study, PCR amplification and sequencing of bacterial 16S
rRNA genes with universally conserved rDNA primers has identified
over 524 unique bacterial sequences affiliated with 12 bacterial
divisions. The molecular sequences exhibited less than 5% similarity
in bacterial community composition between seawater and the healthy,
black band diseased, and dead coral surfaces. Clone libraries from
the BBD bacterial mat were comprised of eight bacterial divisions and
13% unknowns. Several sequences representing bacteria previously
found in other marine and terrestrial organisms (including humans)
were isolated from the infected coral surfaces.
Interestingly enough, although the filamentous cyanobacterium in the
BBD mat has been previously optically identified as Phormidium
corallyticum (Rutzler and Santavy, 1983) there is no sequence in
GenBank for P. corallyticum. This has been puzzling because a 1995
abstract suggests that P. corallyticum had been isolated and
sequenced (Santavy, Schmidt, and Wilkinson, 1995; Phylogeny of
Phormidium corallyticum using 16S rRNA, 7th Annual Symposium on
Environmental Releases of Biotechnology Products, ERL GB S665).
Please contact me directly if you would like to receive a preprint or
have any further questions.
Best regards, Bruce
Bruce W. Fouke, Ph.D.
Department of Geology
University of Illinois
1301 W. Green Street
Urbana, IL 61801 USA
Office Phone: (217) 244-5431 Office FAX: (217) 244-4996
Lab Phones: (217) 333-0672 or (217) 244-9848
Email: fouke at uiuc.edu
Web Site: http://www.geology.uiuc.edu/~bfouke/
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