Chlorophyll extraction using methanol
alark at mail.usyd.edu.au
Tue Sep 17 23:41:56 EDT 2002
The most comprehensive work on methanol extraction of chlorophyll has
been done by Bob Porra in Canberra. A summary of this work is in:
Porra RJ 1991. Recent advances and re-assessments in chlorophyll
extraction and assay procedures for terrestrial, aquatic and marine
organisms including recalcitrant algae. In "The Chlorophylls" H.
Scheer, ed., pp 31-57 CRC Press, Boca Raton.
This lists all the absorption coefficients and equations for
chlorophyll determination. Up to that time, and as far as I know
since, there are no trichromatic equations for determining Chls a+b+c
in methanol (or for determing Chl a+c). The only equations available
are those of Jeffrey and Humphrey, for 90% acetone.
>Has anyone tried or know of any literature where chlorophyll was
>extracted from coral tissue using methanol to get relative or absolute
>comparisons? If so what were the absorbance wavelengths used? I
>have looked extensively through the coral literature and would greatly
>appreciate any help.
>Thank you for your time.
>Department of Biology
>Georgia Southern University
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Prof. A.W.D. Larkum
Acting Director, Sydney University Biological Informatics and
Technology Centre (SUBIT)
School of Biological Sciences
Heydon-Laurence Building (A08)
University of Sydney
Tel 61 2 9351 2069
Fax 61 2 9351 4119
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