[Coral-List] tissue separation

Selina Ward S.Ward at marine.uq.edu.au
Mon Aug 25 20:18:30 EDT 2003

Dear Muthu
I decalcify using hydrochloric acid and formalin.  I use acid that is made
for use in swimming pools as this is inexpensive. In 1 litre I would use
900ml water, 80ml acid, 20ml formalin.  This is gentle enough to keep
reproductive structures intact and, for small finger sized pieces, takes
about a week with one change of acid. This of course varies according to the
Selina Ward

Dr Selina Ward
Australian Coral Reef Society.
Centre for Marine Studies
The University of Queensland
St Lucia 4072 Qld
ph 61 7 3346 9576
mob 61 410 478467
fax 61 7 33654755
email s.ward at marine.uq.edu.au

-----Original Message-----
From: shashank Keshavmurthy [mailto:iamshanky15 at yahoo.com]
Sent: 26 àâãóñòà 2003 ã. 1:48
To: bmuthuraman at lycos.com; Coral-List at coral.aoml.noaa.gov
Subject: [Coral-List] tissue seperation

hi muthu
tissue seperation form the skeleton involves
dissolving the calcium....so mild acid will do...
it is also important to know the purpose for the
tissue seperation.
for histology, I decalcify the tissue in 1:1
mixture of 20% citric acid and 50% formic acid...
it works very well for me.....but it takes long
time......it gives best result if you keep for
overnight with changes every 3-4 hours.....
best of luck for ur work

"the role of infinitely small in nature is infinitely large"-Louis Pasteur

Keshavmurthy Shashank
Kochi University, Faculty of Agriculture
Lab. of AQUa. Environ. Sci. (LAQUES)
Otsu 200, Monobe, Nankoku-shi
783-8502, Kochi, Japan
alt. id: shashank at cc.kochi-u.ac.jp

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