[Coral-List] RE: Optimal fixatives for coral tissues

shashank Keshavmurthy iamshanky15 at yahoo.com
Wed Jan 14 19:34:00 EST 2004

Hi all 

this is becoming interesting...this is the second
time in a year that this subject has appeared on
the list.....

the use of fixatives and decalcification method
is difficult to be generalised....it varies form
coral species.....

I have been using 4% formalin-Seawater and later
10% formalin-Seawater mixture for fixing and
then, store it in ethanol indefinitely after 48
hours in 10% formalin-seawater.

For decalcification, I prepare 1:1 mixture of 20%
citric acid and 50% formic acid, which works very
fine for me.....i get the tissues decalcified

I agree with James......there is no optimal
method ...one has to decide which method is best
for the  samples he or she is dealing with


"the role of infinitely small in nature is infinitely large"-Louis Pasteur                

Keshavmurthy Shashank
Kochi University, Faculty of Agriculture
Lab. of AQUa. Environ. Sci. (LAQUES)
Otsu 200, Monobe, Nankoku-shi
783-8502, Kochi, Japan
alt. id: shashank at cc.kochi-u.ac.jp
phone: 81 090 8285 9012

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