[Coral-List] re: Seeking method for analyzing DIC drawdown in calcification
Gattuso, Jean-Pierre
gattuso2 at obs-vlfr.fr
Mon Jul 5 11:51:29 EDT 2004
At 16:00 +0000 24/06/04, coral-list-request at coral.aoml.noaa.gov wrote:
> I am attempting to quantify the DIC drawdown
>attributable to calcification of coral skeleton
>in corals from Bermuda. The goal is to have
>corals in closed aquaria with filtered
>circulating water and 13C tagged DIC. Right now
>the biggest problem I am encountering is
>acquiring only the coral skeleton that has taken
>up the tagged DIC. Does anyone know of a way to
>isolate only the most recently grown skeleton
>WITHOUT also including coral skeleton that did
>not take up the tagged DIC? I will be using a
>GC Mass Spec (Europa) to analyze the 13C of the
>skeleton. Any help here would be greatly
>appreciated!
>
>Justin Ossolinski
Justin:
You may find the following method useful:
Reynaud-Vaganay S., Gattuso J.-P., Cuif J.-P.,
Jaubert J. & Juillet-Leclerc A., 1999. A novel
culture technique for scleractinian corals:
application to investigate changes in skeletal
18O as a function of temperature. Marine Ecology
Progress Series 180: 121-130.
A pdf copy is available on my web site at the address shown below.
jp
--
Jean-Pierre Gattuso | mailto:gattuso at obs-vlfr.fr
| http://www.obs-vlfr.fr/~gattuso
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