[Coral-List] re: Seeking method for analyzing DIC drawdown in calcification

Gattuso, Jean-Pierre gattuso2 at obs-vlfr.fr
Mon Jul 5 11:51:29 EDT 2004

At 16:00 +0000 24/06/04, coral-list-request at coral.aoml.noaa.gov wrote:
>     I am attempting to quantify the DIC drawdown 
>attributable to calcification of coral skeleton 
>in corals from Bermuda.  The goal is to have 
>corals in closed aquaria with filtered 
>circulating water and 13C tagged DIC.  Right now 
>the biggest problem I am encountering is 
>acquiring only the coral skeleton that has taken 
>up the tagged DIC.  Does anyone know of a way to 
>isolate only the most recently grown skeleton 
>WITHOUT also including coral skeleton that did 
>not take up the tagged DIC?  I will be using a 
>GC Mass Spec (Europa) to analyze the 13C of the 
>skeleton.  Any help here would be greatly 
>Justin Ossolinski


You may find the following method useful:

Reynaud-Vaganay S., Gattuso J.-P., Cuif J.-P., 
Jaubert J. & Juillet-Leclerc A., 1999. A novel 
culture technique for scleractinian corals: 
application to investigate changes in skeletal 
18O as a function of temperature. Marine Ecology 
Progress Series 180: 121-130.

A pdf copy is available on my web site at the address shown below.



Jean-Pierre Gattuso | mailto:gattuso at obs-vlfr.fr 
| http://www.obs-vlfr.fr/~gattuso

Biogeosciences, a new open access journal: http://www.biogeosciences.net

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