[Coral-List] Chlorophyll questions

[Mark Warner]

> Does it make any difference to use 90% Acetone or
> 100% Acetone to extract chlorophyll form
> zooxanthellae?

It makes a slight difference only in the equations that you use based on the
work of Jeffrey and Humphrey (1975) and Humphrey (1979). The equations are
as follows:

For dinoflagellates & cryptomonads,
Solvent 90% Acetone:
Chlorophyll a = (11.43 E664)-(0.40 E630)
Solvent 100% Acetone:
Chlorophyll a = (11.43 E663)-(0.64 E630)

Just about any question you have regarding micro algal pigments and their
quantification may be found in "Phytoplankton pigments in oceanography"
1997. Eds. S.W. Jeffrey, R.F.C. Mantoura & S.W. Wright. UNESCO publishing

Cheers,
Mark
-----------------------------------
Mark E. Warner
Assistant Professor
University of Delaware
Graduate College of Marine Studies
700 Pilottown Rd.
Lewes, DE 19958
USA

Phone: 302-645-4365
Fax:  302-645-4028