[Coral-List] Alizarin staining
richmond at hawaii.edu
Thu Sep 23 15:29:59 EDT 2004
According to the paper by Austin Lamberts in the Coral Reefs: Research Methods book (UNESCO,1978):
use a concentration of ca. 15 ppm (0.0015%); he suggests making up a stock of 4 - 5 mg of stain per ml of distilled water and diluting this "to taste."
For a coral like Pocillopora damicornis we've had good luck putting colonies into a cooler with aeration, the above concentration (should look like dark cherry kool aid) and allow the corals to be in direct sunlight for 4 - 6 hours. To prevent temperature stress, you can put in a cool pack or two, or use an aquarium inside of a water table with good flow (like a heat exchanger).
Robert H. Richmond, Ph.D.
Kewalo Marine Laboratory
Pacific Biomedical Research Center
University of Hawaii at Manoa
41 Ahui Street
Honolulu, Hawaii 96813
e-mail: richmond at hawaii.edu
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