[Coral-List] Helicostoma sp. culturing

Richard A. Snyder rsnyder at uwf.edu
Tue Sep 6 21:27:28 EDT 2005

Dear Shashank:

If the organism is feeding on Symbodinium in places where it is 
found, then a rice grain+ seawater will not likely work.  That method 
is OK for messy cultures of bacterivores.  You need to get a culture 
of the Symbodinium or related dino, culture it, wash the algal medium 
away (filtration or centrifugation) to remove the ammonium.  Use aged 
open ocean seawater (best), 0.2 mm filtered.  If you don't have aged 
seawater, get the cleanest ocean water you can find.  Let it sit for 
a month in a 50 L or larger carboy if you can, longer is better, 
don't disturb the sediment in the bottom.  Filter or autoclave the 
water.  Add F/2 concentrate for dino culture.  Reverse bubbling in 
separatory  funnels with a grow light works well.  Instant ocean may 
work but kills some protists.  Try isolating individual cells of the 
ciliate by micropipette into suspensions of washed dinos.  12 well 
tissue culture plates work well since you can start multiple clonal 
cultures in the same plate, keep track of them under a stereo 
scope,and add more dinos and resuspend sedimented dinos as necessary. 
You may contact me with questions if you think I can help.

Good luck.


>Today's Topics:
>    1. Helicostoma sp.  culturing.... (shashank Keshavmurthy)
>Message: 1
>Date: Mon, 22 Aug 2005 04:40:46 -0700 (PDT)
>From: shashank Keshavmurthy <iamshanky15 at yahoo.com>
>Subject: [Coral-List] Helicostoma sp.  culturing....
>To: coral-list at coral.aoml.noaa.gov
>Message-ID: <20050822114046.21052.qmail at web31805.mail.mud.yahoo.com>
>Content-Type: text/plain; charset=iso-8859-1
>Dear All,
>Does anyone know how ciliate Helicostoma sp. can
>be cultured in lab??? i mean what media is
>I was wondering using a sea water media with
>grain of rice in it...
>any suggestions is welcome....
>"the role of infinitely small in nature is infinitely large"-Louis
>Keshavmurthy Shashank
>phD candidate
>Kochi University, Graduate School of Kuroshio Science
>Laboratory of Environmental Conservation
>Otsu 200, Monobe, Nankoku-shi
>783-8502, Kochi, Japan
>alt. id: shashank at cc.kochi-u.ac.jp
>phone: 81 090 8285 9012
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>End of Coral-List Digest, Vol 26, Issue 16


Richard A. Snyder, Ph.D.
Associate Professor
Center for Environmental Diagnostics and Bioremediation (CEDB)
Biology Department
University of West Florida
11000 University Parkway                                (850)  474-2806
Pensacola, FL  32514                                    FAX:          -3130

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