[Coral-List] Chlorophyll Fluorescence with the WALZ Diving PAM - Request for Suggestions
Jeremy J. Sofonia
jeremy at sofonia.com
Wed Apr 4 09:27:39 EDT 2007
My colleagues and I are currently preparing to use PAM - chlorophyll
fluorometry as a method of detecting potential sub-lethal affects to
Scleractinian corals. In this, we may have up to 300 individual
colonies across multiple locations. My concerns at this stage
primarily involve the dark adaptation of so many individual
colonies. Literature research, so far has shown that timeframes for
dark adaptation may range from hours to minutes, to no adaptation
whatsoever. My previous experience using PAM is fairly limited,
mostly in laboratory conditions where we could just flick the switch.
- Can anyone who is familiar with in situ PAM assessment provide a
suggestion on typical time requirements for dark adaptation?
- Is dark adaptation a requirement or can other levels of YIELD still
provide an good indication of photosynthesis, fluorescence, and/ or
- Any tricks for dark adapting colonies of various morphologies? Sizes?
We're working our way through our sampling design, but I thought that
tapping the expertise of 'The List' may be a good idea. Any
assistance would be greatly appreciated.
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