[Coral-List] Coral cell culture
andiachaves at gmail.com
Fri Apr 6 16:46:37 EDT 2007
I had been culture coral cells from Duncanopsamia sp., Galaxea sp.,
Acropora sp. and Siderastrea radians. I used as a culture medium M199
in most cases. To separate zooxanthellae you can use a Percoll
Gradient. It´s a little hard separate all the zooxanthellae from coral
cells but I think that is possible. In some of my gradients I could
clean samples and get coral cells only with few zooxanthellae (but it
wasn´t my objective take out all of them). For Percoll Gradient
technique or Ficol Gradient (other compound that you can use) check
some papers of sponge cells research:
Pomponi, S.A. and Willoughby, R. 1994. Sponge cell culture for
production of bioactive metabolites. In Proceedings of the 4th
International Porifera Congress: Sponge in Time and Space, Amsterdam,
the Netherlands, 19-23 April 1993. Edited by RWM Van Soest, Th.M.G.
van Kempen and J.C. Braekman. A.A. Balkema, Rotterdam. pp. 395-400.
Richelle-Maurer, E. Gomez, R. Braekman, J-C. Van de Vyver, G. Van
Soest, R.W.M. and Devijver, C. 2003. Primary cultures form the marine
sponge Xestospongia muta (Petrosiidae, Haplosclerida). J. Biotechnol.
The pH for cultures medium or other solutions that you use has to be
close to 8.0.
For your coral cell cultures using different medium I recommended to
review the thesis of Andrea Shaw:
Shaw, A. 2004. Cellular interactions between a pathogenic fungus and a
gorgonian coral host. College of Agriculture and Life Sciences.
MSc. Biología Marina
Universidad Nacional de Colombia
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