[Coral-List] zooxanthellae culture

[Lucia Bongiorni]

Dear Coral-List,

I am trying to set up culture of zooxanthellae from Acropora in silica free F/2 media, in an incubation chamber provided with a 50W- light /dark cycle (12/12h ) at ca.26°C, but I'm facing problems with diatoms contamination (beside  silica is not present in the media) and very slow growth of algae. I would very much appreciate any suggestions on isolation and culturing zooxanthellae from Acropora

thanks in advance 
Lucia Bongiorni


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Lucia  Bongiorni Ph.D
Marine Biology and Ecology Lab
Department of Marine Science
Polytechnic University of Marche
Via Brecce Bianche, 60131, Ancona, ITALY
TEL:  +39 071 2204294
FAX:  +39 071 2204650
email: l.bongiorni at univpm.it