[Coral-List] Workshop in 3D imaging and protein tracking at University Western Sydney, 16-19 November
A.Salih at uws.edu.au
Wed Oct 6 23:19:08 EDT 2010
Tracking Molecules with Light
You are invited to attend the 3rd Advanced Bio-Imaging Workshop at the University of Western Sydney on 16 - 19 November, 2010 and the Bio-Imaging Expo (free event on 16th November)
Training focussed on confocal microscopy and protein 3D tracking, aggregation, diffusion analyses in live samples. Many of the experiments will involve live coral and anemone tissues and cells, symbiotic and other marine algae as well as cultured mammalian cells and plant samples. Experiments will involve studies of protein migration in and between cellular compartments and organelles, changes due to apoptosis in healthy and stressed cells, as well as fluorescence lifetime imaging of photosynthetic function in vivo.
Registration at www.uws.edu.au/3rd_advanced_bio-imaging_workshop.
Places limited to 35 and only 20 places left so register now.
Location: Confocal Bio-Imaging Facility
Hawkesbury Campus, University of Western Sydney
Organiser: Dr Anya Salih
Training by: Dr Salih UWS;
Prof. E. Gratton and Dr M. Digman Univ. California;
Prof. Guy Cox Uni Sydney;
Dr Wolfgang Becker, Becker & Hickl GmbH;
Dr C Thoni Leica, G. Symonds Zeiss
Lectures and intensive hands-on training on confocal microscopes by top researchers in the field. Learn how to explore and analyse the 3D structural complexity of invertebrate, animal & plant cells, tissues and micro-organisms, visualize and analyse movement of organelles and molecules. Trial a range of novel GFP-type protein constructs. Discuss your experiments and trial new approaches. Workshop emphasis on advanced confocal imaging techniques - FRET, FRAP, FCS, RICS, N&B, FLIM, photoactivatable fluorescent proteins.
Professor Enrico Gratton, Director Laboratory for Fluorescence Dynamics, University of California, Irvine
Professor Takeharu Nagai, Laboratory for Nanosystems Physiology & Nikon Imaging Center, Hokkaido University Research Institute Electronic Science, Japan
Dr. Michelle Digman, Director Optical Biology Core Facility, University of California, Irvine
A/Professor Guy Cox, Australian Centre for Microscopy & Microanalysis, University of Sydney
Professor Leann Tilley, Department of Biochemistry, D/Director Centre of Excellence in coherent X-ray Science, La Trobe University
Dr Will Hughes, Director, Pieter Huveneers Molecular Imaging Facility, Garvan Institute of Medical Research, University of Sydney
Dr Louise Cole, Advanced Microscopy Facility, Bosch Institute, University of Sydney
Dr Wolfgang Becker, Director Becker & Hickl GmbH, Berlin
Training sessions cover the following:
Multi-colour Fluorescent proteins
Genetically encodable GFP-type proteins (EGFP, YFP, CFP, mRuby, pmKate2 from Evrogen), fused to studied proteins (mitochondrial, H2B histone, actin, tubulin, Golgi, membrane); Photoactive Fluorescent Proteins in live coral cells as well as protein fusions (EosFP, kindling proteins, Phamret) in mammalian cells. Biosensors - HypPer (Evrogen), Ca2+. Studies of protein localization & diffusion.
Confocal Spectral Imaging
Acquisition of microspectral data (x, y, lambda) in 3D image stacks from samples with multiple fluorescent probes or from fluorescent coral tissues expressing a variety of GFP-type proteins (A. Salih fluorescent corals http://www.abc.net.au/science/articles/2010/08/16/2984168.htm?topic=health)- Spectral unmixing, analysis, spectral FRET.
Analysis of molecular movement and diffusion
Track proteins and other molecules in live cells. Measure protein femtoliter concentrations. Monitor mobility and binding using fluorescence correlation spectroscopy (FCS), scanning FCS, raster image correlation spectroscopy (RICS), number & brightness (N&B) and photon counting histogram (PCH).
Fluorescence Lifetime Imaging Microscopy (FLIM)
- a powerful tool to analyse spatial distribution of excited state lifetimes in samples: studied examples will include FRET-FLIM to study protein interactions (e.g., DNA-Protein) in cells, imaging of photoactivatable FPs, quenching of chlorophyll in plants, etc. Hands-on training in FLIM and Phasor FLIM.
Workshop microscopes & companies:
Leica TCS SP5 (two systems) - UV, VIS and IR in one system, acousto optical beam splitter (AOBS), spectral imaging, FLIM, FCS, RICS, N&B
Zeiss LSM 780 confocal - 32-channel GaAsP array, spectral imaging, photon counting, FCS
Nikon A1, Coherent Scientific Pty. Ltd - rapid image acquisition, resonant scanner & 32 channel microspectral detection
Olymus FluoView FV1000 - variable barrier filter (VBF), spectral detection, RICS and N&B
Ultra VIEW VoX spinning disc confocal microscope, high speed, multichannel, 2D and 3D, FRAP, FLIP, photoactivation experiments, PerkinElmer.
Confocal FLIM system, Becker & Hickl GmbH, Berlin
A range of new GFP-type protein constructs of many colours (cyan to far red) linked to a variety of cellular proteins for in vivo protein localization and dynamic studies, Evrogen
And many more other instruments.
Full workshop registration (lectures + training) will be limited to 35 participants.
Registration will be on a first come first served basis.
Contact Anya Salih a.salih at uws.edu.au to reserve your workshop place
Attendance of lectures only - unlimited in numbers but need to register.
Students - $650 (GST inclusive)
All other - $850 (GST inclusive)
Attendance of the BioImaging Expo on 16th November does not require registration by please rsvp Pamela McMurtry [pamela.mcmurtry at theconferenceteam.com.au]
Accommodation details at registration website, from $260 per week per single room.
Bus shuttle will be available between Sydney airport and the workshop venue at UWS campus.
Registration deadline 1 November, 2010.
61 2 45701452
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