[CDHC] Interesting articles

[Eric Borneman]

Dear list:

I recently came across the following articles, most of them from an 
older text edited by Howard Lenhoff. There are some interesting 
histological and culture techniques here for Hydra.  I do not know if 
they would be applicable to corals, but might be worth investigation. 
Apologies if any of you are already aware of them, and would appreciate 
any feedback from the histologists or cell culture folks if they have 
been tried and were successful or not.


Greber, Martin J., Charles N. David, and Thomas W. Holstein. 1992. A 
quantitative method for separation of living Hydra cells. Roux's Arch 
Dev Biol 201: 296-300.

The following are all from Lenhoff, Howard M. 1983. Hydra: Research 
Methods. Plenum Press, NY.  ISBN 0306410869

Stiven, Alan E. Experimental methods for epizootiological research with 
Hydras.

Pardy, R.L. Preparing aposymbiotic Hydra.

Rutherford, Charles L.  Special techniques for weighing microgram 
quantities of tissue and assaying them for enzyme activities.

Muscatine, L. Isolating endosymbiotic algae from Hydra viridis.

Campbell, Richard D. Preparing histological sections for light 
microscopy.

Day, Robert M. and Howard M. Lenhoff. Isolating mesolamellae.

Marcum, Beverly A. and Richard D. Campbell. Eliminating all 
nonepithelial cells using colchicine.

Lesh-Laurie, Georgia E. Separating viable tissue layers.

David, Charles N. Dissociating Hydra tissue into single cells by the 
maceration technique.

Dunne, John E. and C. Lynne Littlefield. Vital staining: fluorescent 
and immunofluorescent, and review of nonfluorescent dyes.

Campbell, Richard D. Whole mounts for light microscopy.




Best,

Eric Borneman