Chlorophyll extraction

[Joshua Feingold]

Carrie - I've not used methanol, but I did use 95% acetone for chlorophyll
extractions directly from coral tissues without preliminary extraction of
the zooxanthellae. Obviously, this method would only allow correction for
coral surface area and reveal nothing about chlorophyll/zoox. Extraction is
performed in the dark following methods of Jeffrey & Haxo 1968 (in
Biological Bulletin). Wavelengths of 480, 630, 665 and 750 nm were used.
Results were corrected for extract volume and coral surface area to yield
amount of pigment per cm^2. The equation for Chlorophyll a (ug cm^2) =
((11.43 x E665 - 0.40 x E630) x volume of extract) / area of coral. The
equation for Chlorophyll c (ug cm^2) = ((27.09 x E630 - 3.63 x E665) x
volume of extract) / area of coral.

Check that reference for information on Methanol extraction.

Cheers,

Joshua Feingold

At 11:39 AM 09/16/2002 -0400, cmackich at email.gasou.edu wrote:
>Has anyone tried or know of any literature where chlorophyll was
>extracted from coral tissue using methanol to get relative or absolute
>comparisons?  If so what were the absorbance wavelengths used?  I
>have looked extensively through the coral literature and would greatly
>appreciate any help.
>Thank you for your time.
>
>Carrie MacKichan
>Department of Biology
>Georgia Southern University
>
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