No subject

[gefenyov at post.tau.ac.il]

hello to you all
i'm using a fluorescence prob to detect bacteria inside stone corals.
i'm checking crushed tissue sampels and cross sections (10 microns depth)of
the
coral.
i have a serious problem of auto fluorescence of both crushed and whole
sampels
on the same wavelength i'm using for my probs (490, 630 and 540 nm).
the auto fluorescence is produced by the algea and by the coral tissue
itself.
i'm looking for a technique to decrease the auto fluorescence using
prehybridization washing or some other method.
thanks

Yuval Gefen
department of molecular microbiology
Tel-aviv university, Israel.
gefenyov at post.tau.ac.il

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