[Coral-List] RE: Optimal fixatives for coral tissues

[shashank Keshavmurthy]

Hi all 

this is becoming interesting...this is the second
time in a year that this subject has appeared on
the list.....

the use of fixatives and decalcification method
is difficult to be generalised....it varies form
coral species.....

I have been using 4% formalin-Seawater and later
10% formalin-Seawater mixture for fixing and
then, store it in ethanol indefinitely after 48
hours in 10% formalin-seawater.

For decalcification, I prepare 1:1 mixture of 20%
citric acid and 50% formic acid, which works very
fine for me.....i get the tissues decalcified
overnight....

I agree with James......there is no optimal
method ...one has to decide which method is best
for the  samples he or she is dealing with

Regards
Shashank 


=====
"the role of infinitely small in nature is infinitely large"-Louis Pasteur                

Keshavmurthy Shashank
Kochi University, Faculty of Agriculture
Lab. of AQUa. Environ. Sci. (LAQUES)
Otsu 200, Monobe, Nankoku-shi
783-8502, Kochi, Japan
alt. id: shashank at cc.kochi-u.ac.jp
phone: 81 090 8285 9012

__________________________________
Do you Yahoo!?
Yahoo! Hotjobs: Enter the "Signing Bonus" Sweepstakes
http://hotjobs.sweepstakes.yahoo.com/signingbonus