[Coral-List] Alizarin staining

[Richard E. Dodge]

Note that alizarin can cause a significant depression in calcification ......
According to:
Dodge, RE; Wyers SC; Frith, HR; Knap, AH; Smith SR; Cook, CB; and Sleeter, 
TD. Coral calcification rates by the buoyant weight technique: effects of 
Alizarin staining, J. Exp. Mar. Biol. Ecol. 1984. 75. 217-232.

Good Luck Too,
Dick

At 05:29 9/24/2004 +1000, Robert Richmond wrote:
>According to the paper by Austin Lamberts in the  Coral Reefs: Research 
>Methods book (UNESCO,1978):
>
>use a concentration of ca. 15 ppm (0.0015%); he suggests making up a stock 
>of 4 - 5 mg of stain per ml of distilled water and diluting this "to taste."
>For a coral like Pocillopora damicornis we've had good luck putting 
>colonies into a cooler with aeration, the above concentration (should look 
>like dark cherry kool aid) and allow the corals to be in direct sunlight 
>for 4 - 6 hours.  To prevent temperature stress, you can put in a cool 
>pack or two, or use an aquarium inside of a water table with good flow 
>(like a heat exchanger).
>Good luck,
>Bob
>Robert H. Richmond, Ph.D.
>Research Professor
>Kewalo Marine Laboratory
>Pacific Biomedical Research Center
>University of Hawaii at Manoa
>41 Ahui Street
>Honolulu, Hawaii 96813
>Phone: 808-539-7331
>Fax: 808-599-4817
>e-mail: richmond at hawaii.edu
>
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Richard E. Dodge, Ph.D.
Nova Southeastern University Oceanographic Center
8000 N. Ocean Drive
Dania, FL 33004
954-262-3651
954-262-4020 fax
NSUOC http://www.nova.edu/ocean/
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