[Coral-List] Coral List Post
Jennifer Hester
jenmhester at hotmail.com
Tue Nov 15 12:54:36 EST 2005
Hello,
I am an undergraduate student at the University of Hawaii at Hilo and
am working on my senior thesis. My project involves the genotyping of
zooxanthellae collected from coral samples around the island of
Hawaii. I am having difficulties in the succcessful PCR amplification
of the ITSI and ITSII regions of zooxanthellae from Porites lobata
samples. I have tried annealing temperature gradients and template
dilution (1/100), but with no success. Does anyone have any hints or
suggestions as to how to get the zooxs from Porites to amplify? Any
help or information would be greatly appreciated.
Thanks,
Jennifer Hester
Senior Thesis Student
University of Hawaii at Hilo
[1]jenmhester at hotmail.com
(561)909-7631
References
1. mailto:jenmhester at hotmail.com
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