[Coral-List] Using the Diving PAM
Steve Dalton
sdalton at nmsc.edu.au
Thu Oct 20 03:44:29 EDT 2005
I am currently setting up a lab experiment to monitor photosynthetic
efficiency of a subtropical coral species at different temperature. In
addition I will be monitoring corals affected by disease to determine if
zooxthanthellae and coral tissue in front of the disease margin are
stressed prior to death using a Walz diving PAM. Having not used the
diving PAM before, I was wondering if anyone could provide a protocol that
is appropriate in determining fluorescence yield and relative election
transfer. I am planning to place fragments of Turbinaria frondens into
glass aquariums located in a controlled environment room where the
temperature is set a 21 degrees, and light is provided by 10000K and
actinic four foot tubes. Treatments for the experiment include 21 degrees
(control), 24, 26 (temp where T. frondens bleach in situ), and 28 degrees
aquariums in which individual fragments will be placed. Four fragments
located in separate beakers in each aquarium will be monitored for
photosynthetic efficiency 1, 2, 4 and 8 hours and 1, 2, 4 and 8 days after
the experiments starts. I will hopefully be measuring the maximum quantum
yield (dark adapted) and effective quantum yield for each sample during
each specified time. Not sure if I have to measure maximum yield before
each effective yield measurement, could you please advise. Also I wish to
determine electron transfer rates and produce rapid light curves.
Also, wondering if the standard PAM settings are appropriate to use for
this experiment, where the fragments are not subjected to normal in situ
light intensities and the correct distance to place the optic fiber from
the sample. The PAM manual suggest 10mm, however, related publications
generally use 5mm.
If anyone can provide me with an idea of how to use the PAM correctly or
point me to publications that describe the correct use of the PAM your
guidance is sincerely appreciated.
Regards
Steven Dalton
PhD student
University of New England
NMSC Postgraduate Representative
National Marine Science Centre
Bay Drive, Charlesworth Bay
(PO Box J321)
Coffs Harbour, NSW Australia 2450
Ph: 6648 3928
sdalton at nmsc.edu.au
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