[Coral-List] Using the Diving PAM

Steve Dalton sdalton at nmsc.edu.au
Thu Oct 20 03:44:29 EDT 2005

I am currently setting up a lab experiment to monitor photosynthetic 
efficiency of a subtropical coral species at different temperature. In 
addition I will be monitoring corals affected by disease to determine if 
zooxthanthellae and coral tissue in front of the disease margin are 
stressed prior to death using a Walz diving PAM.  Having not used the 
diving PAM before, I was wondering if anyone could provide a protocol that 
is appropriate in determining fluorescence yield and relative election 
transfer.  I am planning to place fragments of Turbinaria frondens into 
glass aquariums located in a controlled environment room where the 
temperature is set a 21 degrees, and light is provided by 10000K and 
actinic four foot tubes.  Treatments for the experiment include 21 degrees 
(control), 24, 26 (temp where T. frondens bleach in situ), and 28 degrees 
aquariums in which individual fragments will be placed.  Four fragments 
located in separate beakers in each aquarium will be monitored for 
photosynthetic efficiency 1, 2, 4 and 8 hours and 1, 2, 4 and 8 days after 
the experiments starts.  I will hopefully be measuring the maximum quantum 
yield (dark adapted) and effective quantum yield for each sample during 
each specified time.  Not sure if I have to measure maximum yield before 
each effective yield measurement, could you please advise.  Also I wish to 
determine electron transfer rates and produce rapid light curves.
Also, wondering if the standard PAM settings are appropriate to use for 
this experiment, where the fragments are not subjected to normal in situ 
light intensities and the correct distance to place the optic fiber from 
the sample.  The PAM manual suggest 10mm, however, related publications 
generally use 5mm.
If anyone can provide me with an idea of how to use the PAM correctly or 
point me to publications that describe the correct use of the PAM your 
guidance is sincerely appreciated.


Steven Dalton
PhD student
University of New England
NMSC Postgraduate Representative
National Marine Science Centre
Bay Drive, Charlesworth Bay
(PO Box J321)
Coffs Harbour, NSW Australia 2450
Ph: 6648 3928
sdalton at nmsc.edu.au

More information about the Coral-List mailing list