[Coral-List] Coral cell culture

[Gary K. Ostrander]

Natalia,

The following papers should address your concerns:

Kopecky, L.J. and Ostrander, G.K.  1999.  Isolation and primary culture of 
viable multicellular endothelial isolates from hard corals.  In Vitro 
Cellular & Developmental Biology. 35:616-624.

Domart-Coulon, I.J., Ebert, D.C., Scully, E.P., Calimlim, P.S. and 
Ostrander, G.K.  2001.  Aragonite crystallization in primary cell cultures 
of mult-cellular isolates from a hard coral, Pocillopora damicornis. 
Proceedings of the National Academy of Sciences, USA   98:11885-11890.


Scully, E.P. and Ostrander, G.K.  2001.  Laboratory models for the study of 
coral pathologies.   Hydrobiologia.  460:91-95.



Best,



GKO



************************************
Gary K. Ostrander  Ph.D.
Interim Dean
John A. Burns School of Medicine
Vice Chancellor for Research
     and Graduate Education
2500 Campus Road  Hawaii Hall 211
University of Hawaii
Honolulu, HI  96822
808/956-7837 office
808/956-2751 fax
*********************************
----- Original Message ----- 
From: "Natalia Jimenez" <natalia7ivonne at yahoo.com.mx>
To: <coral-list at coral.aoml.noaa.gov>
Sent: Thursday, April 05, 2007 5:08 AM
Subject: [Coral-List] Coral cell culture


Dear Coral-list Members:

  I am trying to establish a coral cell culture using DMEM, GMIM and M199 
mediums for my thesis, but I have some questions about it, I would like to 
know if the coral cell can survive without the zooxanthella in those mediums 
and whether there is a technique to separate the zooxanthella from the coral 
cell without damage the last one (inhibition of the photosynthesis process 
by darkness could be a possibility?). I also would like to know which pH is 
the best for the culture and a likely treatment to isolate the coral cell 
from the zooxanthella after separation.

  I would appreciate very much if someone can help me with some information 
about those items.

  Thank you in advance.

  Kind regards.

  Natalia Ivonne Vera Jiménez
  Marine Biologist (UJTL, Colombia)
  MSc Animal Sciences student (WUR, The Netherlands)


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