[Coral-List] zooxanthellae culture

Daniel Poland dpoland at buffalo.edu
Sat Mar 24 10:11:39 EDT 2007

Hi Lucia,

a standard way to reduce diatom growth is to add germanium dioxide to the
f/2 media. In addition, you can streak algal cultures out on petri dishes
with f/2 agar media, then pick individual colonies of zooxanthellae from the
plate (may take awhile to grow). Good luck!


-----Original Message-----
From: coral-list-bounces at coral.aoml.noaa.gov
[mailto:coral-list-bounces at coral.aoml.noaa.gov] On Behalf Of Lucia Bongiorni
Sent: Friday, March 23, 2007 2:04 PM
To: coral-list at coral.aoml.noaa.gov
Subject: [Coral-List] zooxanthellae culture

Dear Coral-List,

I am trying to set up culture of zooxanthellae from Acropora in silica free
F/2 media, in an incubation chamber provided with a 50W- light /dark cycle
(12/12h ) at ca.26°C, but I'm facing problems with diatoms contamination
(beside  silica is not present in the media) and very slow growth of algae.
I would very much appreciate any suggestions on isolation and culturing
zooxanthellae from Acropora

thanks in advance 
Lucia Bongiorni

Lucia  Bongiorni Ph.D
Marine Biology and Ecology Lab
Department of Marine Science
Polytechnic University of Marche
Via Brecce Bianche, 60131, Ancona, ITALY
TEL:  +39 071 2204294
FAX:  +39 071 2204650
email: l.bongiorni at univpm.it
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