[Coral-List] zooxanthellae culture

Ranjeet Bhagooli coral at scientist.com
Sun Mar 25 12:46:19 EDT 2007

Hi Lucia and Daniel,

I'm also struggling to get good clean cultures/clones of Symbiodinium from scleractinian corals.

re-GeO2 suggestion - "Thomas et al (1978) added GeO2 to natural phytoplankton samples in an attempt to separate flagellate productivity from diatom productivity and noted that dinoflagellate photosynthesis was inhibited as well." Thus it does not seem that good to add GeO2 in cultures of Symbiodinium if they are to be used for physiological experiments, unless the culturing intention is otherwise.

reference: Thomas et al (1978) Diatom productivity compared to other algae in natural marine assemblages. J. Phycol. 14:2580-253

May be repeated subculturing after Symbiodinium cells selection under the microscope might help!

Also note that clade D symbiodinium grows very very slowly. And may be the algae you've got in culture could be a member of clade D!

All the best,

> ----- Original Message -----
> From: "Daniel Poland" <dpoland at buffalo.edu>
> To: "'Lucia Bongiorni'" <l.bongiorni at univpm.it>, coral-list at coral.aoml.noaa.gov
> Subject: Re: [Coral-List] zooxanthellae culture
> Date: Sat, 24 Mar 2007 10:11:39 -0400
> Hi Lucia,
> a standard way to reduce diatom growth is to add germanium dioxide to the
> f/2 media. In addition, you can streak algal cultures out on petri dishes
> with f/2 agar media, then pick individual colonies of zooxanthellae from the
> plate (may take awhile to grow). Good luck!
> Daniel
> -----Original Message-----
> From: coral-list-bounces at coral.aoml.noaa.gov
> [mailto:coral-list-bounces at coral.aoml.noaa.gov] On Behalf Of Lucia Bongiorni
> Sent: Friday, March 23, 2007 2:04 PM
> To: coral-list at coral.aoml.noaa.gov
> Subject: [Coral-List] zooxanthellae culture
> Dear Coral-List,
> I am trying to set up culture of zooxanthellae from Acropora in silica free
> F/2 media, in an incubation chamber provided with a 50W- light /dark cycle
> (12/12h ) at ca.26°C, but I'm facing problems with diatoms contamination
> (beside  silica is not present in the media) and very slow growth of algae.
> I would very much appreciate any suggestions on isolation and culturing
> zooxanthellae from Acropora
> thanks in advance
> Lucia Bongiorni
> ***********************
> Lucia  Bongiorni Ph.D
> Marine Biology and Ecology Lab
> Department of Marine Science
> Polytechnic University of Marche
> Via Brecce Bianche, 60131, Ancona, ITALY
> TEL:  +39 071 2204294
> FAX:  +39 071 2204650
> email: l.bongiorni at univpm.it
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Ranjeet Bhagooli, Ph.D.
University of Miami
4600 Rickenbacker Causeway
Miami, Florida 33149

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