[Coral-List] new 454 transcriptomes: Acropora hyacinthus , Porites astreoides
matz at mail.utexas.edu
Fri Jul 2 20:51:00 EDT 2010
In accord with our policy of rapid share of the next-gen sequencing
data, we are happy to announce the release of 454-sequenced
transcriptomes for two corals, Acropora hyacinthus (larvae) and
Porites astreoides (adult), sequenced with 454-titanium and assembled
with the latest version of Newbler (Roche).
We also created a new assembly for Acropora millepora (larvae).
Although it is based on the same reads as previous versions, the new
assembler seem to have performed a lot better.
We are currently using these datasets to scan for signatures of
positive natural selection on coral genes. If you have the same
interest, we would appreciate if you let us know before using our
data. Otherwise, the data are free for everyone to use.
Download the assembled and annotated contigs here:
If you need raw reads with quality scores for your own assembly, just
let us know. If this is this case, we hope that you will follow our
philosophy and release your assembly at once - we and other coral
molecular biologists could always use more sequence coverage!
Mikhail "Misha" Matz
University of Texas at Austin
Integrative Biology Section
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