[Coral-List] Tracking molecules with light, Sydney Australia - scholarships for students
A.Salih at uws.edu.au
Sat Oct 30 19:24:08 EDT 2010
TRACKING MOLECULES WITH LIGHT - Last chance to register
WHERE: University of Western Sydney, Hawkesbury, NSW Australia
WHEN: 16 - 19 November, 2010.
The Workshop is for students and researchers at various levels of experience with little prior training in confocal imaging or those interested in learning the more advanced techniques. A range of live samples will be used, including mammalian, invertebrate (coral, anemones, etc) cells and tissues, plants and algae, marine biofilms and bacteria.
You will be trained in the standard confocal techniques in 2D, 3D and 4D analyses, time-lapse imaging and interactive volume rendering. You will also be trained in the advanced techniques, focusing on protein localization and movement in cells; the analyses of their association with each other; concentrations, diffusion rates and lifetimes in sub-cellular compartments or in an entire cell.
Students AU$650 (GST inclusive)
All other AU$850 (GST inclusive)
Accommodation: UWS College next to workshop venue AU $220 per week
Transportation and all light meals during workshop are covered by registration fees.
SCHOLRASHIPS for GRADUATE STUDENTS:
Becker & Hickl company, who made fluorescence lifetime imaging methods available to laboratories world-wide, are providing 4 scholarships to graduate students to attend the workshop. Evidence of enrolment for a higher degree study must be provided at registration. Scholarships will be awarded on first come first served basis so reserve your place now. Flights and accommodation fees are not covered. Contact Workshop organiser Anya Salih for further information a.salih at uws.edu.au, TEL. 61245701452.
.. Professor Takeharu Nagai, Laboratory for Nanosystems Physiology & Nikon Imaging Center, Hokkaido University Research Institute Electronic Science, Japan
.. Professor Enrico Gratton, Director Laboratory for Fluorescence Dynamics, University of California, Irvine
.. A/Professor Guy Cox, Australian Centre for Microscopy & Microanalysis, University of Sydney
.. Professor Leann Tilley, Department of Biochemistry, La Trobe University
.. Dr Will Hughes, Director, Molecular Imaging Facility, Garvan Institute of Medical Research, University of Sydney
.. Dr Louise Cole, Advanced Microscopy Facility, Bosch Institute, Univ Sydney
.. Dr. Michelle Digman, Director Optical Biology Core Facility, University of California, Irvine
.. Dr Wolfgang Becker, Director Becker & Hickl GmbH, Berlin
.. Dr Mihail Matz, University of Texas, Austen
.. Dr Anya Salih, University of Western Sydney, Australia
Training sessions cover the following:
Cellular structural analysis in 2D, 3D and 4D
Confocal imaging of live cells, dynamic tracking over time, imaging of cellular organelles and alterations during stress.
Multi-colour fluorescent proteins
Jelly-fish derived and coral GFP-type proteins fused to proteins of interest (e.g., mitochondrial, H2B histone, actin, tubulin, Golgi, membrane proteins); applications of novel Photoactive Fluorescent Proteins (EosFP, AmilRFP, kindling proteins) from reef corals to track cellular events.
Confocal Spectral Imaging
Acquisition of microspectral data in 3D image stacks from samples with multiple fluorescent probes or from fluorescent coral tissues expressing a variety of GFP-type proteins.
Analysis of molecular movement and diffusion
Track proteins and other molecules in live cells. Measure protein femtoliter concentrations in sub-cellular compartments. Monitor mobility and binding using fluorescence correlation spectroscopy (FCS) etc.
Fluorescence Lifetime Imaging Microscopy (FLIM)
- a powerful tool to analyse spatial distribution of excited state lifetimes at every pixel of a sampled image: studied examples will include FRET-FLIM to study protein interactions (e.g., DNA-Protein) in cells and coral tissues, imaging of photoactivatable FPs, quenching of chlorophyll in symbiotic algae, etc.
Workshop microscopes & companies
Leica TCS SP5 - two confocal microscopes with light and multiphoton lasers
Zeiss LSM 780 Confocal
Nikon A1 Confocal
Olymus FluoView FV1000
Ultra VIEW VoX spinning disc confocal microscope,
Confocal FLIM system, Becker & Hickl GmbH
Zeiss laser micro-dissection microscope for gene sampling
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