[Coral-List] zooxanthellae clades
Alejandro tatis serrano
tatisserrano87 at gmail.com
Mon Feb 4 08:32:39 EST 2013
Good afternoon, my name is Alejandro Tatis, i´m a biology undergraduate
student at the University of Magdalena, Santa marta, Colombia. For my
Bachelor thesis I´m working with *Acropora palmata* and *A. cervicornis *
zooxanthellae.* *I have made many PCR using 28S primers especific to
zooxantellae, including 28s2 forward 5’-CCT CAG TAA TGG CGA ATG AAC A-3’
and 28s2 reverse 5’-CCT TGG TCC GTG TTT CAA GA-3’ (Loh et al.,2001) , as
well as the digestión with Taq I restricción enzime. However, I couldn´t
find any paper that let me interpret the RFLPs bands. I wish to know if
anybody knows any reference or paper, as well as figures that would allow
me to discriminate zooxantellae clades on the RFLPs bands.
Thanks a lot!
More information about the Coral-List