[Coral-List] RNA Extraction for coral expertise needed

[Ana Paula B. Moreira]

Dear Sara,

It might be RNAse contamination in your lab.
Maybe try another room.
Best,
Ana Paula

Em sexta-feira, 7 de agosto de 2015, Sara Campana <sc9 at hw.ac.uk> escreveu:

> Hello,
>
> I'm working on gene expression with the coral Stylophora pistillata.
> I developed a procedure to extract the RNA with Qiagen Kit which has been
> working on more than 50 samples, but suddenly in the last three days none
> of my samples seem to have any RNA.
> I'm following the exact same procedure, but the results are almost nil.
> I've been going through a series of experiment to exclude possible
> problems: I tried to use fresh instead of frozen samples to test if the
> storage could be affecting the samples, I tried to use a different
> homogenization method, I tried to work in a different lab in cooler
> conditions, but nothing seems to be working anymore.
> I tried my procedure with a sample from mussel and it did work perfectly,
> the problem seems to be confined to coral tissue and I can't think about
> any other cause.
>
> If someone had similar issues or have further suggestion, please let me
> know.
>
> Many thanks,
> Sara Campana.
> sc9 at hw.ac.uk <javascript:;>
>
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-- 
Ana Paula B. Moreira, *PhD*
PDJ - CNPq
˜˜˜˜˜˜˜˜˜˜˜˜˜˜˜˜˜˜˜˜˜˜˜˜˜˜˜
Lab. de Microbiologia - BIOMAR
UFRJ
Av. Carlos Chagas Fo., 373
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