[Coral-List] tissue seperation

shashank Keshavmurthy iamshanky15 at yahoo.com
Mon Aug 25 11:47:35 EDT 2003

hi muthu
tissue seperation form the skeleton involves
dissolving the calcium....so mild acid will do...
it is also important to know the purpose for the
tissue seperation.
for histology, I decalcify the tissue in 1:1
mixture of 20% citric acid and 50% formic acid...
it works very well for me.....but it takes long
time......it gives best result if you keep for
overnight with changes every 3-4 hours.....
best of luck for ur work

"the role of infinitely small in nature is infinitely large"-Louis Pasteur                

Keshavmurthy Shashank
Kochi University, Faculty of Agriculture
Lab. of AQUa. Environ. Sci. (LAQUES)
Otsu 200, Monobe, Nankoku-shi
783-8502, Kochi, Japan
alt. id: shashank at cc.kochi-u.ac.jp

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