[Coral-List] tissue seperation

[shashank Keshavmurthy]

hi muthu
tissue seperation form the skeleton involves
dissolving the calcium....so mild acid will do...
it is also important to know the purpose for the
tissue seperation.
for histology, I decalcify the tissue in 1:1
mixture of 20% citric acid and 50% formic acid...
it works very well for me.....but it takes long
time......it gives best result if you keep for
overnight with changes every 3-4 hours.....
best of luck for ur work
cheers
shashank  


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"the role of infinitely small in nature is infinitely large"-Louis Pasteur                

Keshavmurthy Shashank
Kochi University, Faculty of Agriculture
Lab. of AQUa. Environ. Sci. (LAQUES)
Otsu 200, Monobe, Nankoku-shi
783-8502, Kochi, Japan
alt. id: shashank at cc.kochi-u.ac.jp

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