[Coral-List] critical tech note about 454
mikhail matz
matz at mail.utexas.edu
Sun Jul 19 12:24:51 EDT 2009
Hello colleagues:
to all of you doing 454 and/or SOLiD sequencing: please be aware
(unless you already are) that these methods cannot directly sequence
material extracted from an agarose gel that was stained with EtBr and
imaged under UV. Emulsion PCR of such material may look great, but
the number and quality of the reads will be very bad. The solution is
to use gels stained with SYBR Safe and imaged under blue light.
cheers
Misha
Mikhail V. Matz
University of Texas at Austin
Integrative Biology Section
1 University station C0930
Austin, TX 78712
phone 512-992-8086 cell, 512-475-6424 lab
fax 512-471-3878
web http://www.bio.utexas.edu/research/matz_lab
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